我正在使用一个称为raxml的系统发育软件,而我wnat为每个Phylip文件构建单个树。对于具有三个Phylip文件的目录,我进行了以下
##files in directory
Ortho1.phy Ortho6.Phy Ortho6.Phy
for f in /home/Single_trees/trimmed_alignment/*.phy; do raxmlHPC -f a -x 100 -m PROTGAMMAAUTO -p 100 -s $f -N 100 -n $f.tree; done;
但这总是给我一个错误,说不允许$符号。
raxmlHPC: axml.c:5236: analyzeRunId: Assertion `0' failed.
错误字符/在运行ID中不允许
有更好的方法吗?我尝试使用此链接使用此链接在此处使用作业数组https://rc.fas.harvard.edu/resources/documentation/submittit-large-numbers-numbers-obs-jobs-jobs-to-odyssey/它。
这是我尝试的数组作业提交的方法:
#!/bin/bash -l
#
# raxml.sbatch
#
#SBATCH -J consensus # A single job name for the array
#SBATCH -p high # best partition for single core small jobs
#SBATCH -n 12 # one core
#SBATCH -N 1 # on one node
#SBATCH -t 100:00:00 # Running time of 2 hours
#SBATCH --mem 18000 # Memory request of 4 GB
#SBATCH -o raxml_%A_%a.out # Standard output
#SBATCH -e raxml_%A_%a.err # Standard error
module load raxml
for FILES in /home/aligned_fasta/.phy; do
echo ${FILES}
done;
# grab out filename from the array exported from our 'parent' shell
FILENAME=${FILES[$SLURM_ARRAY_TASK_ID]}
# make & move into new directory, and run!
mkdir ${FILENAME}_out
cd ${FILENAME}_out
raxmlHPC -f a -x 100 -m PROTGAMMAAUTO -p 100 -s $FILENAME -N 100 -n $FILENAME.tree
#Now, we grab all the appropriate files and submit them en-batch with an array:
# grab the files, and export it so the 'child' sbatch jobs can access it
export files =($(LS -1 .phy))
# get size of array
NUMPHY=${#FILES[@]}
# now subtract 1 as we have to use zero-based indexing (first cell is 0)
ZBNUMPHY=$(($NUMPHY - 1))
# now submit to SLURM
if [ $ZBNUMPHY -ge 0 ]; then
sbatch --array=0-$ZBNUMPHY raxml.sbatch
fi
我使用sbatch -array = 0-10 raxml.sh提交,但它不起作用。
刚刚发现了一些东西。基本上,我将重命名文件,以便它们是连续的,并且可以使用slurm。
ls *.phy | cat -n | while read num file; do mv $file ${file/./.$num.}; done
,文件将为
Ortho1.1.phy Ortho6.2.Phy Ortho6.3.Phy
然后,您可以按照以下方式进行操作:
#!/bin/bash -l
# SBATCH -J tree
###### Standard out and Standard Error output files with the job number in the name.
#SBATCH -o tre_%A.%a.out
#SBATCH -e tre_%A.%a.err
###### number of nodes
###SBATCH --nodes=6
###SBATCH --nodes=6
###### number of processors
#SBATCH -n 16
###SBATCH --cpus-per-task=4
###### Spread the tasks evenly among the nodes
####BATCH --ntasks-per-node=8
###### coupled with array
####SBATCH --ntasks=1-179
#SBATCH --time=300:00:00
#SBATCH -p high
#SBATCH --mem 24000
###### Want the node exclusively
### SBATCH --exclusive
#SBATCH --array=1-3
module load raxml
for i in $SLURM_ARRAY_TASK_ID.phy
do
echo $i
done
### tree
raxmlHPC-PTHREADS -f a -x 100 -m PROTGAMMAAUTO -p 100 -T 16 -s $i -N 100 -n $i.tree
这将为您提供单独的树,您可以用来建造共识树。
我认为您可能正在引导并获得共识系统发育,在这种情况下,RAXML中有一个特殊情况可以做到这一点。当我有分钟时,我会在这里发布。